FIELD: medicine. SUBSTANCE: recombinant plasmid DNA pVR6-II3 containing 3027 nucleotide pairs is prepared for specific detection of rhotaviruses of subgroup II. It contains fragment of DNA plasmid dVR6-II3 of size 281 nucleotide pairs which shows homology with site of sixth gene of human rhotavirus of subgroup II (size is 753-1034 nucleotide pairs), fragment of vector plasmid pGEM4z (size is 2746 nucleotide pairs). Also, strain of bacterium - a carrier of fragment of DNA dVR6z for specific detection of rhotaviruses of subgroup II is a subject of invention. EFFECT: preparing of recombinant plasmid DNA indicated above.
Authors
Dates
1995-03-27—Published
1991-12-11—Filed