FIELD: biotechnology. SUBSTANCE: method involves preparing DNA fragment from the natural plasmid from Escherichia coli H 104407 which is used for pathogenic enterobacteria detection containing colony-enhancing factor CFA 1. Fragment size is 950 nucleotide pairs being nucleotides 1-484 were located in noncoding part of operon CFA 1 and nucleotides 485-950 in encoding part of gene cfa B. This fragment is used as DNA-probe for detection of genes of operon CFA 1 and for indication of enterobacterium strains containing colony-enhancing factor CGA 1. EFFECT: preparing DNA fragment indicated above. 1 tbl
Authors
Dates
1995-12-10—Published
1992-12-21—Filed