STRAIN MICROMONOSPORA PURPUREA VAR VIOLACEA - A PRODUCER OF ANTIBIOTIC GENTAMYCIN, AND A METHOD OF GENTAMYCIN PRODUCING Russian patent published in 1995 - IPC

FIELD: biotechnology, antibiotics. SUBSTANCE: vegetative inoculum of culture strain-producer Micromonospora purpurea var. violacea deposited in Collection ВНИИА at number 1931 is grown for two stages. At the first stage aqueous nutrient medium of the following composition is used, %: potato starch 2.4-2.6; pea flour 1.0-1.2; corn extract 0.6-0.8; chalk 0.4-0.5; at the second stage aqueous nutrient medium of the following composition is used, % : potato starch 2.5-3.5; glucose 0.15-0.3; soybean flour 1.5-2.5; ammonium sulfate 0.25-0.32; sodium chloride 0.25-0.35; chalk 0.4-0.6; cobalt chloride hexahydrate 0.00006-0.00016. Strain fermentation is carried out on the nutrient medium of the following composition, %: potato starch 4.5-5.2; glucose 0.15-0.3; soybean flour 3.2-4.0; ammonium sulfate 0.15-0.3; sodium chloride 0.25-0.35; chalk 0.5-0.8; cobalt chloride hexahydrate 0.00008-0.0002, and water - the rest. Gentamycin concentration in cultural fluid reaches 2100 mcg/ml. EFFECT: increased yield of antibiotic. 3 cl