FIELD: biotechnology. SUBSTANCE: method involves milling of pancreas-containing organs of terrestrial and aqueous animals, extraction of proteolytic complex with 1 mM calcium chloride solution containing 0.2% detergent. Then extract is centrifuged, and supernatant is subjected for microfiltration. Proteolytic complex is isolated by ultrafiltration through diaphragms retaining capability of which is 15000-20000 Da. EFFECT: improved method of proteolytic complex preparing.
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF TREATING INFECTED WOUND | 1992 |
|
RU2036658C1 |
| FISH CAVIAR PRODUCTION METHOD | 1993 |
|
RU2043738C1 |
| METHOD OF PREPARING OF ENZYME PREPARATION OF PROTEOLYTIC ACTION FROM THE VISCERA OF FRESH OR FROZEN FISH | 1985 |
|
RU1339917C |
| METHOD FOR PREPARING FRESHLY SALTED CREAM-LIKE PRODUCT OF HYDROBIONTS | 1991 |
|
RU2040189C1 |
| METHOD FOR RECOVERING COMPLEX OF PROTOLYTIC ENZYMES FROM SEA PRODUCTS | 0 |
|
SU1030409A1 |
| METHOD FOR WASTELESS COMPLEX REPROCESSING OF CHITIN-CONTAINING RAW MATERIAL | 2000 |
|
RU2207033C2 |
| METHOD OF PREPARING SALTY GRAINY CAVIAR FROM THE FRESH AND FROZEN FISH ROES | 1994 |
|
RU2060669C1 |
| METHOD FOR ISOLATING COMPLEX OF PROTEOLYTIC ENZYMES FROM PANCREATIC GLAND OF WHALES | 0 |
|
SU981360A1 |
| METHOD FOR MANUFACTURE OF NUTRITIONAL SEMI-FINISHED PRODUCT FROM LAMINARIA ALGAE | 1991 |
|
RU2041656C1 |
| METHOD FOR PREPARING FISH ROE | 1994 |
|
RU2090103C1 |
