FIELD: biotechnology. SUBSTANCE: alkaline phosphatase is purified by extract loading on the chromatographic sorbent, equilibrated with buffer solution of tris-hydrochloride and magnesium chloride. Sorbent is aminopropylated silica (pore diameter is 500-3000 
) with covalent linked chitosan N-sulfosuccinate (0.5-50 mg per 1 ml sorbent) following by step gradient elution with 0.2 M sodium chloride in buffer solution. Specific activity of prepared alkaline phosphatase is 4000-5000 U/mg protein. Product can be used as an enzyme-marker in high-sensitive immunoenzyme assay. EFFECT: high specific activity of prepared enzyme.
| Title | Year | Author | Number |
|---|---|---|---|
| ADSORBENT FOR EXTRACTING ATHEROGENIC LIPOPROTEINS FROM BIOLOGICAL LIQUIDS | 1992 |
|
RU2029564C1 |
| METHOD OF SULFATED CHITOSAN PREPARING | 1992 |
|
RU2048475C1 |
| ADSORBENTS FOR EXTRACTING ATHEROGENIC LIPOPROTEINS FROM BIOLOGICAL LIQUIDS | 1992 |
|
RU2029565C1 |
| METHOD OF PURIFYING ALKALINE PHOSPHATASE | 0 |
|
SU1554377A1 |
| METHOD OF PREPARING INTERPOLYMERIC COMPLEXES OF CHITOSAN WITH SODIUM DEXTRAN SULFATE | 1992 |
|
RU2048473C1 |
| METHOD FOR PREPARING HEPARINS OF LOW MOLECULAR MASS | 2005 |
|
RU2295538C2 |
| METHOD OF ALKALINE PHOSPHATASE PREPARATION | 0 |
|
SU1713265A1 |
| METHOD OF PURIFYING ALKALINE PHOSPHATESE | 0 |
|
SU1426089A1 |
| PROCESS FOR PREPARING PHOSPHOLIPASE D | 0 |
|
SU1125246A1 |
| METHOD FOR ISOLATING POLYNUCLEOTIDE PHOSPHORILASE | 0 |
|
SU1076445A1 |
