FIELD: veterinary. SUBSTANCE: essence of this method resides in preparing botulinic antitoxin vaccine from strain No. 59, type C, having biological activity of about 400 to 600 thousands lethal doses of toxin for white mice (volume: 1.0 cu. cm), adding aluminum potassium sulfate for adsorbing botulinic antigen, treating whole stock with physiological salt solution, and finally admixing inactivated cultures of Pseudomonas aeruginosa of four strains Nos 40 (381-II-06), 48 (1-011), 63 (1677-08) and 77 (Dn 05) in certain proportions at microbial cell concentration of about 8 to 9 bln per 1 cu. cm. EFFECT: disclosed method allows manufacturing sterile biological preparation harmless for animals and possessing high immunogenic activity. 3 tbl
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