STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF RESTRICTION ENDONUCLEASE ECO27K1 Russian patent published in 1995 - IPC

FIELD: biotechnology. SUBSTANCE: product: restriction endonuclease Eco27K1 recognizing and splitting the nucleotide sequence 5′-C!PyCGPuG-3′. Restrictase produced by proposed strain is an isoschizomer of restrictase AvaI and can be used in genetic engineering investigations. Proposed strain Escherichia coli BKM B-2005D is isolated from the clinical isolates and provides the high level of enzyme content 500000 U/g crude matter of cell biomass, not less. The yield of purified enzyme is 30000 U/g cell biomass. EFFECT: increased activity of enzyme proposed.