FIELD: biotechnology. SUBSTANCE: proposed strain of Escherichia coli BKM CR-370D is prepared by transformation with plasmid DNA pBGM3 containing genes of restriction-modification CFRBI in strain E.coli Z85. Strain provides high level of enzyme content, 5 mln. U/g wet matter of cell biomass. The yield of purified enzyme is 200000 U/g cell biomass. EFFECT: increased yield of enzyme.
Authors
Dates
1995-06-27—Published
1992-07-30—Filed