FIELD: biotechnology. SUBSTANCE: product: restriction endonuclease Eco110KI recognizing and splitting the nucleotide sequence 5′-PuGGNC!CPy-3′. Restrictase produced by proposed strain is an isoschizomer of restrictase SauI and can be used in genetic engineering investigations. Proposed strain Escherichia coli BKM B-2004D is isolated from the clinical isolates and provides the high level of enzyme content 1000000 U/g crude matter of cell biomass, not less. The yield of purified enzyme is 20000 U/g cell biomass. EFFECT: increased activity of enzyme.
| Title | Year | Author | Number |
|---|---|---|---|
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|
RU2044053C1 |
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RU2044055C1 |
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|
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| RECOMBINANT PLASMID DNA ECO 1831RI ENCODING SYNTHESIS OF RESTRICTION ENDONUCLEASE ECO 1831RI, STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF RESTRICTION ENDONUCLEASE ECO 1831RI | 1992 |
|
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| METHOD OF OBTAINING ECO RV ENDONUCLEASE RESTRICTION | 1984 |
|
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| STRAIN OF BACTERIA BACILLUS ALVEI - A PRODUCER OF RESTRICTASE BAVI | 0 |
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SU1453896A1 |
