METHOD OF CYCLOSPORINE A PRODUCING, STRAINS OF FUNGUS SESQUICILLIOPSIS ROSARIENSIS G ARNOLD - PRODUCERS OF CYCLOSPORINE A, STRAIN OF FUNGUS TOLYPOCLADIUM INFLATUM - A PRODUCER OF CYCLOSPORINE A Russian patent published in 1996 - IPC

FIELD: microbiological industry, antibiotics. SUBSTANCE: method of cyclosporine A producing involves fermentation of fungus colony of the strain Sesquicilliopsis rosariensis G. ARNOLD IMET 43888 or S. rosariensis G. ARNOLD IMET 43887 or Tolypocladium inflatum IMET 43899 to red or black-brown color, under aerobic conditions, on nutrient medium containing ammonium salts. Medium has additionally citrate (carbon source), trace elements - zinc, iron and/or calcium cations. Culture suspension is mixed with accessory filtering agent - recrystallized gypsum, precosit or calcite flour followed by soaking with methanol or ammonium aqueous solution, extraction with simple carboxylic acid ether or supercritical gas, mainly carbon dioxide. Then extract is defatted and the end product is isolated by chromatography on silica gel and/or aluminium hydroxide or by HPLC. EFFECT: improved method of cyclosporine A production. 10 cl