FIELD: antibiotics, pharmacology of peptides.
SUBSTANCE: invention relates to high-active crystalline preparation of cyclosporine A and a method for its preparing. Invention relates to high-active crystalline preparation of cyclosporine A representing cyclic undecapeptide prepared from fungus of genus Tolypocladium inflatum subsporum blastosporum grown in cultural medium containing additionally rubomycin in the concentration 2 mcg/ml and zinc sulfate in the concentration 5 mg/ml. The fermentation medium comprises maltex and D,L-valine as main components wherein the final concentration of preparation in medium is 2.0-2.5 mg/ml. Product is isolated from fungus dried mycelium by extraction with petroleum ether before and after purification by chromatography method on silica gel and crystallized in system hexane - acetone (4:1). Invention provides the development of effective cyclosporine preparation wherein the content of cyclosporine A is 80-85%.
EFFECT: improved preparing method.
2 cl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| STRAIN OF FUNGUS TOLYPOLADIUM INFLATUM SUBSP BLASTOSPORUM - A PRODUCER OF CYCLOSPORINE A AND A METHOD OF CYCLOSPORINE A BIOSYNTHESIS | 1988 |
|
SU1830947A1 |
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| METHOD OF PREPARING IMMUNODEPRESSANT CYCLOSPORIN A AND A STRAIN TOLYPOCLADIUM SPECIES CBS 630 92 PRODUCING CYCLOSPORIN A | 1993 |
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