FIELD: medicine, biochemistry. SUBSTANCE: method involves enzymatic hydrolysis of defibrinized calf blood followed by isolation of the end product by ultrafiltration, concentration and sterilizing filtration. Enzymatic hydrolysis is carried out using bacterial endoprotease, enzyme inactivation is carried out by reaction mixture heating to 80-98 C and inert material at molecular mass above 5000-8000 Da is removed by step-by-step micro- and ultrafiltration. EFFECT: improved method of preparing.
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Authors
Dates
1996-09-27—Published
1993-10-12—Filed