FIELD: analytical biochemistry, antibiotics. SUBSTANCE: patient blood sample is treated with mixture consisting of 4.2 ml HEPES-buffer (pH = 7.8), peptidylpropyl-cis-trans-isomerase (specific activity is 500 mole/min x mg, molecular mass is 17 kDa), 0.1 mg Glt-Ala-Ala-Pro-phenylalanine-4-nitroanilide and incubated at 5 C for 20 min followed by spectrophotometry at 410 nm. Cyclosporine A concentration is calculated using calibration plot. Sensitivity of method is 50 ng/ml blood. Method is used in laboratory diagnosis. EFFECT: improved method of assay.
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