METHOD OF CHYMOPAPAIN ADDITIONAL PURIFICATION Russian patent published in 1997 - IPC

FIELD: biotechnology, pharmacy. SUBSTANCE: method involves preparing the parent solution of chymopapain, passing unpurified solution through column with affinity chromatography matrix exhibiting specificity to chymopapain active site and backing that is bound by covalent linkage with N-terminal end of chymopapain-inhibitory peptide through the spacer group. C-terminal amino acid of chymopapain-inhibitory peptide is a derivative of phenylalanine, alanine, cyclohexylalanine or leucine that is bound with chymopapain active site more firmly than with PPIV active site. The indicated peptide is bound to chymopapaine molecule active site. Elution is carried out by suitable eluent at pH = 1.2-1.8 followed by separation of ballast proteins, neutralization of eluate and its desalting by dialysis, passing through column with the indicated matrix for affinity chromatography, eluation of chymopapain with suitable eluent, combining active fractions, their dialysis and lyophilic drying. EFFECT: improved method of purification. 4 cl, 10 tbl