METHOD FOR SETTING INTRAVITAL LYSTERIOSIS DIAGNOSIS Russian patent published in 1997 - IPC

FIELD: medicine. SUBSTANCE: method involves applying indirect solid phase enzymoimmune assay by using enzymoimmune assay lysteriosis antigen in 1:2000 dilution degree followed by sensibilizing polystyrene plates by means of this antigen during 16-18 h at 20-22 C and its coating with 1% serum antibody solution under condition of 1 h exposure at 37 C and following 3-fold washing and subsequent layering the serum under investigation using 1 h exposure time, 3-fold washing and subsequent layering conjugate for 1 h with following 3-fold washing and introducing substratum mixture for 20-30 min at 20-25 C. Visual or instrumental recording is carried out in conclusion. Intensive coloring of the serum under investigation considerably exceeding intensive coloring of the negative control serum is considered to be positive result pointing to lysteriosis disease to be the case. EFFECT: enhanced accuracy of diagnosis in short time.