FIELD: medicine, biochemistry, immunochemistry. SUBSTANCE: alcoholic deposit is precipitated with 16% polyethylene glycol (molecular mass is 4000-6000) and C5/C3 pool is isolated from the formed precipitate by anion-exchange chromatography on DEAE-Toyopearl and C5-fraction is separated on cation-exchange resin CM-Sephadex and purified additionally by gel-filtration on Sephacryle S-200. Invention can be used in medicine for disease diagnosis associated with complement system activation. EFFECT: simplified method, decreased cost.
Authors
Dates
1997-05-10—Published
1994-07-08—Filed