FIELD: medicine. SUBSTANCE: method involves preabsorbing Polymixin B into palette cells in 5-15 mcg/l concentration and the adding samples, containing the endotoxin. After thermostating, the cells are washed with phosphate salt buffer solution and serum to antivirulent strain of No 347 B. pertussis at the fourth stage of growth is introduced into the cells. Visualization of binding complex is carried out by means of antispecies conjugate in presence of substratum indication mixture. Quantitative determination of pertussoid endotoxin is carried out by means of comparative titering of sample under examination with Branch Standard sample of the B. endotoxin. EFFECT: enhanced accuracy. 2 dwg
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