METHOD OF ANTIBODIES TEST TO CAPSULAR POLYSACCHARIDE Haemophillus influenzae TYPE b Russian patent published in 2008 - IPC G01N33/569 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, specifically to immunology and concerns method of antibodies test to capsular polysaccharide Haemophillus influenzae type b. Substance of invention implies that working cultivations of analysed and reference serum are applied on solid binder with fixed high-purity capsular polysaccharide Haemophilus influenzae type b, as well as marker samples referring as to serums with relative content of IgG antibodies to capsular polysaccharide polyribosylribitolephoaphate Haemophilus influenzae are incubated, isolated from surplus antibodies, added with conjugate in working cultivation, repeatedly incubated at the same parametres, washed from surplus conjugate, added with substrate mixture containing tetramethylbenzidine and hydrogen peroxide in preset values, incubated in the lightproof place. Reagent is added to stop reaction; optical density of analysed and reference serum are tested for antibodies content. Diagram optical density and conditional content of antibodies in marker samples is drawn and used to detect antibodies content in analysed samples, in standard units. Advantage of invention consists in increase of method sensitivity.

EFFECT: increased sensitivity of method of antibodies test to capsular polysaccharide Haemophillus influenzae type b.

2 tbl, 1 dwg