METHOD OF PREPARING AUTOLYZED YEAST CLARIFIED EXTRACT Russian patent published in 1997 - IPC

FIELD: microbiology, medicine, food industry. SUBSTANCE: yeast suspension is subjected for autolysis in the presence of oleic acid for 4-12 h at pH = 6.0-7.5, heated and precipitated is separated. Extract is concentrated by evaporation up to ACB content 8-20%, concentrate is clarified by heating at 90-100 C and dried if necessary. In the process of autolysis after amine nitrogen content attainment to 1.5-3.5 mg/ml proteolytic enzyme can be added to autolyzate (as measured for 4-8 U/1 g ACB.). After clarification extract can be purified by ultra- or microfiltrated additionally. Any proteolytic enzymes that do not form bitter taste product during hydrolysis can be used. For purification diaphragms at pore diameter 0.1-0.4 mcm or retaining substances with molecular mass above 5-10 kDa were used. EFFECT: improved method of preparing. 3 cl, 3 tbl