FIELD: medicine, immunology. SUBSTANCE: extraction of spirituous precipitate B (Cohn's fraction III) is carried out in 10-fold volume of distilled water. For removing lipoprotein fraction chloroform and also sodium chloride were used at final concentration 0.9%, by volume. Precipitation of immunoglobulin fraction from extract is carried out in the presence of 12% polyethylene glycol. Method provides the increased purity of preparation (content of inert total proteins is decreased from 3-5% in prototype to 0-1%), specific activity is increased by 2-3-fold, the yield - by 1.5-fold and net cost - by 25%. EFFECT: enhanced purity, specific activity and yield. 1 tbl
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Authors
Dates
1997-07-20—Published
1994-07-08—Filed