FIELD: biotechnology, genetic and protein engineering. SUBSTANCE: method involves culturing the strain-producer Escherichia coli VKPM B-6511 on rich medium. The grown cells were disrupted, hybrid protein ILOX-3 is isolated, split with enzymes, C-terminal lysine residue is replaced with amido-group and the end product is obtained. Recombinant plasmid DNA pTOTEilox-3 encoding hybrid protein ILOX-3 for oxytocin preparing, molecular mass is 2.34 mDa, consists of Hind III/Eco RI-fragment of plasmid TOTE2IL-3 containing tandem of promoters P8 of phages fd and ptac, two lac-operators, enhancer of gene 10 phage T7 translation, terminator of phage fd transcription, β-lactamase gene and N-terminal part of human interleukin-3, and Hind III/Eco RI-fragment containing synthetic gene of oxytocin oligomer with 3 monomeric units flanked with lysine residues. Strain of bacterium Escherichia coli VKPM B-6511 - a producer of hybrid protein ILOX-3 is obtained by transformation of cells Escherichia coli TG1 with plasmid pTOTEilox-3. EFFECT: improved method of oxytocin producing. 3 cl
