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2 113 483

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IPC

(21) (22)

Application

96113021/13, 1996-06-21

(22)

Actual filing date

1996-06-21

(45)

Published

1998-06-20

(72)

Inventor

Korobko V.G.Shingarova L.N.Petrovskaja L.E.Petrenko L.A.Pustoshilova N.M.

(73)

Holder

Gosudarstvennyj Nauchnyj Tsentr Virusologii I BiotekhnologiiInstitut Bioorganicheskoj Khimii Im.M.M.Shemjakina I Ju.A.Ovchinnikova

RECOMBINANT PLASMID DNA PGGF-8 ENCODING POLYPEPTIDE SHOWING PROPERTIES OF HUMAN GRANULICYTIC COLONY-STIMULATING FACTOR AND THE STRAIN OF BACTERIUM ESCHERICHIA COLI - PRODUCER OF POLYPEPTIDE SHOWING PROPERTIES OF HUMAN GRANULOCYTIC COLONY-STIMULATING FACTOR Russian patent published in 1998 - IPC

Abstract RU 2113483 C1

FIELD: biotechnology, genetic engineering. SUBSTANCE: recombinant plasmid DNA has synthetic gene encoding human granulocytic colony-stimulating factor, promoter tandem of tryptophan operon of E.coli and synthetic enhancer of gene-10 translation of bacteriophage 17. Strain Escherichia coli as a producer of this polypeptide is obtained. Biosynthesis of polypeptide shows constitutive character and its level is 10% of total cellular protein content, not less. Invention can be used for preparing human granulocytic colony-stimulating factor. EFFECT: recombinant plasmid DNA and strain of bacterium indicated above, increased production of factor. 2 cl, 4 dwg

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RU 2 113 483 C1

Authors

Korobko V.G.

Shingarova L.N.

Petrovskaja L.E.

Petrenko L.A.

Pustoshilova N.M.

Dates

1998-06-20—Published

1996-06-21—Filed