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2 144 957

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IPC

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Application

99104063/13, 1999-02-26

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Start date

1999-02-26

(22)

Actual filing date

1999-02-26

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Published

2000-01-27

(72)

Inventor

Korobko V.G.Boldyreva E.F.Shingarova L.N.Vul'Fson A.N.Kostromina T.I.Miroshnikov A.I.Gavrikov V.G.Kalinin Ju.T.Stepanov A.V.

(73)

Holder

Institut Bioorganicheskoj Khimii Im.M.M.Shemjakina I Ju.A.Ovchinnikova RanRossijskoe Aktsionernoe Obshchestvo Otkrytogo Tipa

RECOMBINANT PLASMID DNA PPINS07 ENCODING FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN AND STRAIN OF BACTERIUM ESCHERICHIA COLI - PRODUCER OF FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN Russian patent published in 2000 - IPC

Abstract RU 2144957 C1

FIELD: molecular biology, biotechnology, genetic engineering. SUBSTANCE: invention proposes recombinant plasmid DNA containing artificial gene that encodes the fused polypeptide consisting of a single IgG-bindining domain of protein A from Staphylococcus aureus, peptide linker His₆-Gly-Ser-Arg and human proinsulin, hybrid tac-promoter and terminator of ribosomal operon of E. coli. Invention proposes the strain of Escherichia coli - producer of fused polypeptide showing the human proinsulin sequence. Biosynthesis of fused polypeptide is induced by isopropylthiogalactopyranoside and level of its biosynthesis is 25% of total cellular protein, not less. Invention can be used for preparing the human recombinant proinsulin. EFFECT: increased yield of polypeptide. 3 cl, 2 dwg, 3 ex

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RU 2 144 957 C1

Authors

Korobko V.G.

Boldyreva E.F.

Shingarova L.N.

Vul'Fson A.N.

Kostromina T.I.

Miroshnikov A.I.

Gavrikov V.G.

Kalinin Ju.T.

Stepanov A.V.

Dates

2000-01-27—Published

1999-02-26—Filed