METHOD OF PREPARING MENINGOCOCCUS LIPOPOLYSACCHARIDE OF SEROGROUP B Russian patent published in 1997 - IPC

FIELD: biotechnology. SUBSTANCE: growing is carried out under condition of the controlled culturing on liquid synthetic medium containing the following components, g/l: sodium L-glutamate 1.30 ± 0.5; L-cysteine hydrochloride 0.03 ± 0.02; potassium chloride 0.09 ± 0.08; sodium chloride 6.00 ± 1.00; magnesium sulfate heptahydrate 0.06 ± 0.01; ammonium chloride 1.25 ± 0.9; sodium hydrogen phosphate dodecahydrate 2.5 ± 0.6; sodium citrate 0.50 ± 0.1; glucose 1.60 ± 0.5, and distilled water - up to 1 l. Formalin is added at final concentration 0.2 ± 0.1% to the grown culture N. meningitidis, microbe cells were separated by centrifugation from cultural fluid and the latter is concentrated by ultrafiltration. Cells and concentrate of cultural fluid were dried. Then extraction with phenol aqueous solution is carried out at 62 ± 5 C, the combined aqueous layers were dialyzed and ultracentrifuged three times. Supernatants and deposits were combined and supernatants were treated with 2 ± 1% cetavlon solution. Cetavlon and nucleic acid salts were removed and the purified supernatants were purified finally by gel-chromatography on column with Sephadex G-200 using buffer solution containing sodium deoxycholate. The purified preparation of lipopolysaccharide is lyophilized. EFFECT: increased yield of preparation, improved method. 1 tbl, 2 dwg