FIELD: genetic engineering. SUBSTANCE: method involves cloning of DNA fragment of cattle infectious rhino-tracheitis virus to the vector in which then biotin is incorporated. Single-stranded DNA of phage M13 mp8 is used as a vector, and biotin is used as a label. Biotin is incorporated to vector by chemical DNA modification by cytosine links that ensures to increase the sensitivity of viral DNA detection up to 1-10 pg. EFFECT: increased sensitivity of virus detection. 1 tbl
Authors
Dates
1996-02-20—Published
1991-07-11—Filed