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2 054 487

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Application

5014156/13, 1991-07-11

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Actual filing date

1991-07-11

(45)

Published

1996-02-20

(72)

Inventor

Glotov A.G.Semenikhin V.I.Il'Ichev A.A.Oreshkova S.F.Baturina I.I.Minenkova O.O.Puzyrev A.T.Tikunova N.V.Petrenko V.A.

(73)

Holder

Institut Ehksperimental'Noj Veterinarii Sibiri I Dal'Nego VostokaNauchno-Issledovatel'Skij Konstruktorsko-Tekhnologicheskij Institut Biologicheski Aktivnykh Veshchestv

METHOD OF DETECTION OF CATTLE INFECTIOUS RHINO-TRACHEITIS VIRUS Russian patent published in 1996 - IPC

Abstract RU 2054487 C1

FIELD: genetic engineering. SUBSTANCE: method involves cloning of DNA fragment of cattle infectious rhino-tracheitis virus to the vector in which then biotin is incorporated. Single-stranded DNA of phage M13 mp8 is used as a vector, and biotin is used as a label. Biotin is incorporated to vector by chemical DNA modification by cytosine links that ensures to increase the sensitivity of viral DNA detection up to 1-10 pg. EFFECT: increased sensitivity of virus detection. 1 tbl

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RU 2 054 487 C1

Authors

Glotov A.G.

Semenikhin V.I.

Il'Ichev A.A.

Oreshkova S.F.

Baturina I.I.

Minenkova O.O.

Puzyrev A.T.

Tikunova N.V.

Petrenko V.A.

Dates

1996-02-20—Published

1991-07-11—Filed

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