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Application

98120373/13, 1998-11-12

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Start date

1998-11-12

(22)

Actual filing date

1998-11-12

(45)

Published

2000-01-20

(72)

Inventor

Sorokin A.V.Razumov I.A.Kazachinskaja E.I.Kachko A.V.Ivanova A.V.Mishin V.P.Bukreev A.A.Belanov E.F.Loktev V.B.Netesov S.V.

(73)

Holder

Gosudarstvennyj Nauchnyj Tsentr Virusologii I Biotekhnologii

RECOMBINANT PLASMID DNA PQ-F35 ENCODING FUSED POLYPEPTIDE F35 SHOWING ANTIGENIC AND IMMUNOGENIC PROPERTIES OF MARBURG VIRUS PROTEIN VP 35, METHOD OF ITS PREPARING AND STRAIN OF BACTERIUM ESCHERICHIA COLI - SUPERPRODUCER OF RECOMBINANT POLYPEPTIDE F35 Russian patent published in 2000 - IPC

Abstract RU 2144565 C1

FIELD: molecular biology, biotechnology, genetic engineering, medicine. SUBSTANCE: invention relates to medicinal virology and immunology and can be used for carrying out immunodiagnosis of hemorrhagic Marburg fever and for preparing specific antibodies to Marburg virus and genetic engineering vaccines against Marburg virus. Recombinant plasmid DNA pQ-f35 encoding polypeptide f35 showing antigenic properties of Marburg virus protein VP 35 and recombinant strain of bacterium E. coli JM 103 [pQ-f35] are constructed. This strain provides biosynthesis of this polypeptide at expression level 70 mg of recombinant protein/l of cultural fluid at culturing on liquid nutrient media. Plasmid pQ-f35 has optimized promoter sequence consisting of phage T5 promoter recognized by E. coli RNA-polymerase and two lac-operator sites showing high level of lac-repressor binding and effective expression of the strong phage promoter. EFFECT: enhanced specificity and sensitivity of known methods of immunodiagnosis. 3 cl, 5 dwg, 4 ex

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RU 2 144 565 C1

Authors

Sorokin A.V.

Razumov I.A.

Kazachinskaja E.I.

Kachko A.V.

Ivanova A.V.

Mishin V.P.

Bukreev A.A.

Belanov E.F.

Loktev V.B.

Netesov S.V.

Dates

2000-01-20—Published

1998-11-12—Filed