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2 090 611

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IPC

(21) (22)

Application

96119371/13, 1996-09-27

(22)

Actual filing date

1996-09-27

(45)

Published

1997-09-20

(72)

Inventor

Arzumanov E.N.Arzumanov T.E.Agadzhanjan A.E.Kamzolova S.V.Mel'Nikov V.A.Movsesjan R.A.Sal'Nikova I.V.Samojlenko V.A.Finogenova T.V.Shishkanova N.V.Khachanov D.G.

(73)

Holder

Finogenova Tat'Jana Vasil'EvnaSamojlenko Vladimir AleksandrovichArzumanov Egishe NikolaevichMel'Nikov Vladimir Alekseevich

STRAIN OF YEAST YARROWIA LIPOLYTICA - PRODUCER OF CITRIC ACID, METHOD OF PRODUCING CITRIC ACID AND METHOD OF SODIUM CITRATE ISOLATION Russian patent published in 1997 - IPC

Abstract RU 2090611 C1

FIELD: biotechnology. SUBSTANCE: invention relates to new yeast strain Yarrowia lipolytica VKM Y-2820D. By-side products or waste from ethanol production were used as carbon source for the indicated strain culturing. Process is carried out in fermenter using withdrawal cultural fluid and addition of nutrient medium or in fermenter fitted with diaphragm modulus and continuous or periodical permeate removing. Method of product isolation involves the following stages: removal concomitant ions by solution passing through strong-acid cation-exchange resin in salt form and middle-basic anion-exchange resin in citrate form, decoloration of the obtained solution using clearing ion-sorbent in salt form, evaporation, crystallization and drying. EFFECT: improved method of producing and isolation, new strain indicated above. 6 cl

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RU 2 090 611 C1

Authors

Arzumanov E.N.

Arzumanov T.E.

Agadzhanjan A.E.

Kamzolova S.V.

Mel'Nikov V.A.

Movsesjan R.A.

Sal'Nikova I.V.

Samojlenko V.A.

Finogenova T.V.

Shishkanova N.V.

Khachanov D.G.

Dates

1997-09-20—Published

1996-09-27—Filed