METHOD OF PREPARING THE HETEROLOGIC POLYPEPTIDE IN EUCARYOTIC MICROORGANISMS Russian patent published in 1997 - IPC

FIELD: biotechnology, genetic engineering. SUBSTANCE: method involves construction of recombinant plasmid DNA containing BAR I promoter of Saccharomyces cerevisiae or alcohol dehydrogenase-I promoter of S. cerevisiae, part of gene BAR I sequence encoding signal peptide and point of signal peptide KEX 2 splitting off from heterological polypeptide (the latter is Arg-Arg or Lys-Lys) and structural gene also. Then method involves transformation of the obtained DNA from the strain-recipients, transformants culturing, isolation and purification of the end product. Method proposes the use of the following strain-recipients: S. cerevisiae, Schizosaccharomyces pombe, or Aspergillus, or Neurospora. EFFECT: improved method of preparing. 9 cl, 21 dwg, 5 tbl