FIELD: medical engineering; biological engineering. SUBSTANCE: method involves designing recombinant plasmid pSVpSVγ6969 having DNA fragment encoding predecessor of human immune interferon, transforming COS-7 cells line with the produced recombinant vector, selecting transformed cells that are cultured under conditions providing target product accumulation and selecting mature form of γ-interferon possessing no N-terminal Cys-Tyr-Cys (des-Cys-Tyr-Cys)γ- interferon. EFFECT: enhanced reliability of method. 8 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PREPARING POLYPEPTIDE SHOWING PROPERTY OF HUMAN GAMMA-INTERFERON | 1988 |
|
RU2092561C1 |
| PROCESS FOR PREPARING FABRIC-TYPE PLASMINOGENE ACTIVATOR, STRAIN OF CULTIVATED ANIMAL CELLS, FABRIC-TYPE PLASMINOGENE ACTIVATOR CHO PRODUCER | 1987 |
|
RU2046827C1 |
| THROMBOPOIETIN | 1994 |
|
RU2245365C2 |
| STRAIN OF BACTERIA ESCHERICHIA COLI - A PRODUCER OF PLASMINOGEN ACTIVATOR OF TISSUE TYPE | 0 |
|
SU1662352A3 |
| INTERFERON-LIKE PROTEIN ZCYTO21 | 2001 |
|
RU2292394C2 |
| NOVEL AVIAN CYTOKINES AND GENETIC SEQUENCES CODING THEREOF | 2008 |
|
RU2562108C2 |
| EXPRESSING VECTOR (VARIANTS) | 1995 |
|
RU2177999C2 |
| ISOLATED NUCLEIC ACID ENCODING FELINE LIGAND CD86, DIAGNOSTIC OLIGONUCLEOTIDE, CLONING VECTOR, VACCINE, METHODS FOR INDUCTION OR SUPPRESSION OF IMMUNITY IN CAT | 1999 |
|
RU2263145C2 |
| METHOD OF PREPARING THE HETEROLOGIC POLYPEPTIDE IN EUCARYOTIC MICROORGANISMS | 1986 |
|
RU2091490C1 |
| ANTIBODY AGAINST INTERFERON-α/β-BINDING PROTEIN I (IFNAB-BPI) | 1995 |
|
RU2363705C2 |
