FIELD: biotechnology, genetic engineering. SUBSTANCE: invention relates to a method of preparing genetically modified nuclear polyhedrosis virus Autographa californica that involves effect on virus genome by inactivation of gene encoding enzyme - UDP-glucosyltransferase. The obtained recombinant virus shows the increased effectiveness in insecticide compositions as compared with viruses of wild type or viruses used at the present time. Invention relates to also a method of control over insects that involves applying the genetically modified virus on plants. Method can be used for plant biological protection from pests. EFFECT: improved method of preparing, enhanced effectiveness of protection. 11 cl, 14 dwg , 5 tbl
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