METHOD OF MICROMULTIPLICATION OF STEVIA, STEVIA REBAUDIONA L Russian patent published in 1997 - IPC

FIELD: agriculture, biotechnology. SUBSTANCE: method involves the use of terminal and/or axillary buds with lower stem tissues (size is 1-3 mm) as explant followed by sterilization with 0.1% dioxide solution for 3 min and 0.1% mercury dichloride solution for 2.5 min, culturing for 4-5 weeks on nutrient medium Murasige-Skoog containing phytohormones, indolylacetic acid 0.1-0.3 mg/l, benzyladenine 0.5-2.0 mg/l, gibberellic acid 0.5-2.0 mg/l up to formation of the rooted plant-regenerants, nonrooted regenerants and callus tissue. Then the rooted plants were replanted in soil and the nonrooted regenerants were micrografted and transferred for rooting on the nutrient medium Murasige-Skoog containing 2-6 mg/l indolylacetic acid and 1-2 mg/l gibberellic acid. Callus tissue is placed on the parent nutrient medium for the further culturing up to regenerants obtaining. EFFECT: improved method of micromultiplication. 2 tbl, 7 dwg