FIELD: biotechnology.
SUBSTANCE: invention represents a culture medium for the micropropagation of calciphilic plants in culture in vitro, comprising vitamins and amino acids dissolved in distilled water, on Murashige and Skoog, sucrose in an amount of 20,000 mg/l, agar-agar in an amount of 8,000 mg/l, and also growth regulators 6-benzylaminopurine, gibberellic acid, and 3-indolyl acetic acid, the mineral composition Woody Plant Medium, kinetin, at that the growth regulators are taken in the following concentration, mg/l: 6-benzylaminopurine 0.1-0.3, kinetin 0.9-1.1, gibberellic acid 0.9-1.1, 3-indolyl acetic acid 0.4-0.6.
EFFECT: invention enables to increase the propagation factor by activating the axillary meristems without compromising on the quality of regenerants.
2 tbl
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Authors
Dates
2015-06-10—Published
2014-02-18—Filed