FIELD: biotechnologies.
SUBSTANCE: invention represents a method of reproduction of buckwheat in vitro, comprising a buckwheat reproduction from aseptic seed plantlets. The seeds are pre-sterilised with concentrated sulphuric acid and cultivated in Murashige and Skoog medium without phytohormones. They are subcultivated, using the shoot apex with an auxiliary bud with obtaining of microplants in the nutrient Murashige and Skoog medium with phytohormones containing from 9.2 up to 23.0 mg/l cuprous sulphate, which are separated into quicksets, cultivated, subcultivated and implanted in this medium.
EFFECT: offered method allows to improve efficiency of process of reproduction of buckwheat microplants in conditions in vitro due to its simplification, acceleration, cost saving and gain in yield of lab plants.
3 tbl
| Title | Year | Author | Number |
|---|---|---|---|
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