FIELD: biotechnology, food industry. SUBSTANCE: invention proposes a method of preparing the protein biomass by culturing the strain Pleurotus ostreatus VKPM F-697 on the nutrient medium under aeration condition at pH = 6.0-7.5 followed by biomass separation. Nutrient medium has 0.1-3 vol.% surface-active substance, in part, bone fat or vegetable oil. Sowing material is subjected preliminary for temperature shock at 4-6 C for 4-24 h. EFFECT: high protein content in biomass. 4 cl, 3 tbl
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