FIELD: biotechnology, vaccines. SUBSTANCE: invention relates to preparing highly immune vaccine. Rickettsia (Coxiellae) Bernett accumulation is carried out in murine spleens and their isolation and purification from host tissues is carried out in system of incompatible polymers. Part of the obtained purified corpuscular antigen is used as the first component of vaccine. Other part of corpuscular antigen is subjected for ultrasonic treatment and centrifugation and soluble antigen is obtained as the second component of vaccine. Components 1 and 2 were combined at ratio: 16 mcg Coxiella corpuscles and 0.5 ml soluble antigen at titer 1:10. The prepared vaccine shows high immunogenic activity, prolonged immunity retention at a single vaccination and safety. EFFECT: improved method of preparing, enhanced quality and property.
| Title | Year | Author | Number |
|---|---|---|---|
| INACTIVATED VACCINE AGAINST QUERY FEVER | 1999 |
|
RU2167674C2 |
| STRAIN COXIELLA BURNETTI USED FOR PREPARING OF INACTIVATED VACCINE AGAINST QUERY FEVER | 1991 |
|
RU2026342C1 |
| METHOD FOR PRODUCING A SET OF FLUORESCENT RICKETTSIAL AND QUERY FEVER DIAGNOSTICUMS AND USE THEREOF FOR SEROLOGICAL DIAGNOSIS OF RICKETTSIOSIS AND QUERY FEVER, A SEROLOGICAL DIAGNOSTIC TECHNIQUE | 2019 |
|
RU2728340C1 |
| METHOD FOR ESTIMATING IMMUNIZATION EFFECTIVENESS IN THE CASE OF Q FEVER | 1991 |
|
RU2039982C1 |
| STRAIN OF COXIELLA BURNETTI, USED FOR HYPERIMMUNE SERA PREPARATION | 0 |
|
SU1724683A1 |
| METHOD FOR PREPARING Q FEVER PATHOGEN ANTIGEN | 2003 |
|
RU2236867C1 |
| METHOD OF PREPARING THE SOLUBLE ANTIGEN-BASE VACCINE FOR TICK-BORNE ENCEPHALITIS CONTROL | 1994 |
|
RU2084242C1 |
| METHOD OF BRUCELLOSIS ANTIGEN PREPARING | 1993 |
|
RU2086258C1 |
| METHOD FOR PRODUCING A FLUORESCENT IMMUNOGLOBULIN DIAGNOSTIC AGENT FOR DETECTING PATHOGENS OF RICKETTSIOSES AND COXIELLOSES, FLUORESCENT IMMUNOGLOBULIN DIAGNOSTIC AGENT AND APPLICATION THEREOF | 2021 |
|
RU2769578C1 |
| METHOD OF PREPARING VACCINE AGAINST BRUCELLOSIS | 1991 |
|
RU2054293C1 |
