FIELD: medicine, microbiology.
SUBSTANCE: invention relates to preparing highly active antigen from Bernet's coxiellae for its the following using for diagnosis of Q fever. Method involves homogenization of chicken embryo yolk sacs infected with attenuated strain C. burnetti M-44 and their treatment with ether 1-2 times. Then ether phase is removed and acidified formamide is added to remained mass. In 16-18 h cooled acidified ethanol is added and mixture is centrifuged. Acidified acetone is added to supernatant, then mixture is centrifuged, precipitate is dissolved in physiological solution, pH = 7.2. Method provides preparing highly active antigen of Q fever pathogen with high percent yield of the final product.
EFFECT: improved preparing method.
1 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PREPARING VACCINE FOR Q-FEVER CONTROL | 1994 |
|
RU2094057C1 |
| METHOD FOR PRODUCING A SET OF FLUORESCENT RICKETTSIAL AND QUERY FEVER DIAGNOSTICUMS AND USE THEREOF FOR SEROLOGICAL DIAGNOSIS OF RICKETTSIOSIS AND QUERY FEVER, A SEROLOGICAL DIAGNOSTIC TECHNIQUE | 2019 |
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| METHOD FOR PRODUCING A FLUORESCENT IMMUNOGLOBULIN DIAGNOSTIC AGENT FOR DETECTING PATHOGENS OF RICKETTSIOSES AND COXIELLOSES, FLUORESCENT IMMUNOGLOBULIN DIAGNOSTIC AGENT AND APPLICATION THEREOF | 2021 |
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| STRAIN COXIELLA BURNETTI USED FOR PREPARING OF INACTIVATED VACCINE AGAINST QUERY FEVER | 1991 |
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| 0 |
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