FIELD: biotechnology, medicine, immunology. SUBSTANCE: method involves preparing immune sera containing specific antibodies raised to all basic proteins of antigen to be tested and having different concentration of these antibodies. Different concentration of antibodies in sera is obtained by dilution of native immune sera with dilutant no containing antibodies to the tested antigen, for example, nonimmune serum with stabilizing additions. The most amount of sera were prepared with antibody concentrations similar to their critical ones in sera and that is a border between positive and negative sera; the minimal amount of sera were prepared with antibody concentrations similar to that in native sera. EFFECT: enhanced sensitivity of analysis. 2 cl
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