FIELD: agriculture microbiology, molecular biology, genetic engineering. SUBSTANCE: invention relates to plants protection and offers entomopathogenic strain Bacillus thuringiensis subsp. kurstaki IPM-46 that can be used for development of insecticide biopreparation. Strain is obtained by transfer of recombinant plasmid pTV241 carrying gene Cry3A of B. thuringiensis var. tenebrionis in cells of the strain Bacillus thuringiensis subsp. kurstaki HD73 by electroporation method. New strain synthesizes two insecticide proteins: δ-endotoxin Cry3A and Cry1Ac showing activity with respect of insects of orders Coleoptera and Lepidoptera. This strain exhibits the following advantage as compared with the known ones: high insecticide activity (1.0 x 106 spores/ml) and broadened spectrum of insects exhibiting sensitivity to this strain. Except for representatives of order Lepiroptera with sensitivity to δ-endotoxin Cty1Ac, this spectrum involves Colorado potato beetle with sensitivity to δ-endotoxin as a representative of order Coleoptera. EFFECT: bacterial strain indicated above, enhanced effectiveness of insecticide effect. 1 tbl, 3 ex
| Title |
Year |
Author |
Number |
| RECOMBINANT PLASMID DNA PTV 241, METHOD OF CONSTRUCTION OF RECOMBINANT PLASMID DNA PTV 241, METHOD OF CONSTRUCTION OF PLASMID CONTAINING GENE CRY IIIA IN TRANSPOSON TN 917 CAT, STRAIN OF BACTERIUM BACILLUS THURINGIENSIS SUBSPECIES THURINGIENSIS EXHIBITING ACTIVITY AGAINST COLORADO POTATO BEETLE |
1995 |
|
RU2125091C1 |
| RECOMBINANT PLASMID DNA PBTN 11 DETERMINING SYNTHESIS OF CRYSTALLINE INSECTICIDE DELTA-ENDOTOXIN CRY IIIA-TYPE, METHOD OF ITS CONSTRUCTION AND STRAIN OF BACTERIUM PSEUDOMONAS PUTIDA CONTAINING RECOMBINANT PLASMID DNA PBTN 11 - A PRODUCER OF CRYSTALLINE INSECTICIDE DELTA-ENDOTOXIN CRY IIIA-TYPE SHOWING ACTIVITY AGAINST INSECTS OF COLEOPTERA ORDER |
1995 |
- Dobritsa A.P.
- Koretskaja N.G.
- Kochetkov V.V.
- Svetoch O.E.
- Loseva O.I.
|
RU2103363C1 |
| INCAPSULATED BIOPREPARATION FOR PROTECTING AGAINST COLORADO POTATO BEETLE AND OTHER INJURIOUS INSECTS OF COLEOPTERA TYPE |
2001 |
- Dobritsa A.P.
- Koretskaja N.G.
- Derbyshev V.V.
- Zhigletsova S.K.
- Chubatova S.A.
- Bechina E.M.
|
RU2201679C2 |
| RECOMBINANT PLASMID DNA ENCODING SYNTHESIS OF DELTA-ENDOTOXIN Cry IIIA AND STRAIN BACILLUS THURINGIENSIS SUBSPECIES KURSTAKI PREPARED ON RECOMBINANT PLASMID DNA BASE |
2004 |
- Tjurin Sergej Anan'Evich
- Noskov Kirill Anatol'Evich
- Vejko Vladimir Petrovich
- Meshkov Jurij Ivanovich
- Jakovleva Inna Nikolaevna
- Debabov Vladimir Georgievich
|
RU2278161C1 |
| PREPARATION AGAINST COLORADO POTATO BEETLE AND OTHER COLEOPTEROUS INSECT PESTS |
2001 |
- Dobritsa A.P.
- Derbyshev V.V.
- Krasnova S.P.
- Zhigletsova S.K.
- Chubatova S.A.
- Levchuk V.P.
|
RU2199214C2 |
| STRAIN OF MICROORGANISMS BACILLUS THURINGIENSIS SUBSPECIES KURSTAKI POSSESSING INSECTICIDE-ACARICIDE ACTIVITY AGAINST REPRESENTATIVES OF LEPIDOPTERA, COLEOPTERA, HOMOPTERA, THYSANOPTERA AND ACARIFORMES ORDERS |
2004 |
- Tjurin Sergej Anan'Evich
- Zalunin Igor' Arsen'Evich
- Meshkov Jurij Ivanovich
- Jakovleva Inna Nikolaevna
- Zhuzhikov Dmitrij Pavlovich
- Khashimov Farkhod Khakimovich
- Debabov Vladimir Georgievich
|
RU2278159C1 |
| MUTANT STRAIN OF BACTERIUM BACILLUS THURINGIENSIS SUBSPECIES TENEBRIONIS DSM 5480 - PRODUCER OF DELTA-ENDOTOXIN, METHOD OF DELTA-ENDOTOXIN-BASE PREPARATION PREPARING, DELTA-ENDOTOXIN-CONTAINING PESTICIDE COMPOSITION, METHOD OF CONTROL OF HARMFUL AGRICULTURE INSECTS, METHOD OF PRODUCING MUTANT STRAINS OF BACTERIUM BACILLUS THURINGIENSIS SUBSPECIES TENEBRIONIS - PRODUCERS OF DELTA-ENDOTOXIN |
1990 |
- Khanne Gurtler
- Anette Shousbo Petersen
|
RU2138950C1 |
| ISOLATED AND PURIFIED DNA FRAGMENT CRY IIIC; PROTEIN CRY IIIC TOXIC FOR COLEOPTERA; INSECTICIDE COMPOSITION TO FIGHT AGAINST COLEOPTEROUS INSECTS (VARIANTS); STRAIN OF BACILLUS THURINGIENSIS BACTERIA - A PRODUCENT OF PROTEIN CRY IIIC TOXIC FOR COLEOPTERA (VARIANTS); PROTEIN TOXIC FOR COLEOPTERA; METHOD TO FIGHT AGAINST COLEOPTEROUS INSECTS |
1992 |
- Vill'Jam P.Donovan[Us]
- Mark Dzh.Rupar[Us]
- Annet S.Slejni[Us]
- Timoti B.Dzhonson[Us]
|
RU2106409C1 |
| STRAIN OF BACTERIA BACILLUS THURINGIENSIS SUBSP.KURSTAKI FOR PRODUCING ENTHOMOPATHOGENIC PREPARATION AGAINST EPIDOPTERA AND COLEOPTERA |
0 |
- Tyurin Sergej Ananevich
- Baranov Aleksej Yurevich
- Zalunin Igor Arsenevich
- Pakhtuev Aleksej Ivanovich
- Shashkina Nina Ivanovna
- Komarskikh Renata Pavlovna
- Chegodaev Fedor Nikitovich
- Bauer Lidiya Andreevna
- Antonov Aleksej Arkadevich
- Shimanaev Valerij Anatolevich
- Chestukhina Galina Georgievna
- Stepanov Valentin Mikhajlovich
|
SU1688819A1 |
| METHOD OF POTENTIATION OF PESTICIDE ACTIVITY OF PESTICIDE OBTAINED ON BASIS OF MICROORGANISMS OF GENUS BACILLUS |
1996 |
- Lidster Uil'Jam D.
- Makintosh Suzan K.
- Mehnker Dehnajz K.
- Stanis Robert L.
|
RU2192744C2 |
