RECOMBINANT PLASMID DNA ENCODING SYNTHESIS OF DELTA-ENDOTOXIN Cry IIIA AND STRAIN BACILLUS THURINGIENSIS SUBSPECIES KURSTAKI PREPARED ON RECOMBINANT PLASMID DNA BASE Russian patent published in 2006 - IPC C12N15/00 C12N15/32 C12N15/75 C12N1/15 

FIELD: microbiological industry, biotechnology.

SUBSTANCE: invention relates to production of bacterial preparations. Method involves construction of recombinant plasmid DNA comprising gene determining the resistance to erythromycin (ErmC), sequence of DNA fragment of B. thuringiensis subsp. tenebrionis encoding synthesis of δ-endotoxin cry IIIA, sequence of DNA fragment of chromosome B. thuringiensis subsp. kurstaki of size 448 nucleotide pairs, sequence of DNA fragment of chromosome B. thuringiensis subsp. kurstaki of size 501 nucleotide pairs, and sites for cleavage with restriction site-specific endonucleases Eco RI, Pst I, Bam HI, Hind III and Kpn I. The strain B. thuringiensis subsp. kurstaki comprising the indicated recombinant plasmid DNA is prepared. Invention provides preparing the strain B. thuringiensis possessing the enhanced insecticide activity with respect to representatives of Lepidoptera, Coleoptera and Homoptera orders showing the damage effects on agriculture cultures and crops.

EFFECT: valuable properties of plasmid DNA.

3 cl, 5 dwg, 6 tbl, 4 ex