FIELD: biotechnology, pharmaceutical industry. SUBSTANCE: poly-(A)+-mRNA is isolated from the mitogen-stimulated lymphocytes of peripheral blood and used for cDNA library making. Clones containing plasmid (p67, p68, p69, p70 and p71) with DNA insertion that encodes γ-IFN were obtained; cDNA insertion is isolated from plasmid p69, subjected for sequencing and used for construction of recombinant expression vector pPGK-IFN-g. The latter is used for transformation of the strain Sac. cerevisiae PH 218 followed by selection of transformants and their culturing under conditions providing accumulation of the end protein. The expressed polypeptide showing property of human IFN-g is purified. EFFECT: improved method of preparing. 15 dwg
| Title |
Year |
Author |
Number |
| METHOD FOR PRODUCING HUMAN IMMUNE INTERFERON |
1988 |
- Dehvid V.Goeddel[Us]
- Patrik V.Grej[Us]
|
RU2107728C1 |
| PROCESS FOR PREPARING FABRIC-TYPE PLASMINOGENE ACTIVATOR, STRAIN OF CULTIVATED ANIMAL CELLS, FABRIC-TYPE PLASMINOGENE ACTIVATOR CHO PRODUCER |
1987 |
- David Vannorman Geddel'[Us]
- Vil'Jam Dzhehk Kor[Us]
- Diane Pennika[Us]
- Gordon Allen Vekhar[Us]
|
RU2046827C1 |
| STRAIN OF BACTERIA ESCHERICHIA COLI - A PRODUCER OF PLASMINOGEN ACTIVATOR OF TISSUE TYPE |
0 |
- David Vannorman Geddel
- Vilyam Dzhek Kor
- Diane Pennika
- Gordon Allen Vekhar
|
SU1662352A3 |
| PICHIA PASTORIS YEAST STRAIN - PRODUCER OF CHICKEN INTERFERON-GAMMA, RECOMBINANT PLASMID DNA WHICH FACILITATES BIOSYNTHESIS CHICKEN INTERFERON-GAMMA, AND CONSTRUCTION METHOD THEREOF |
2006 |
- Padkina Marina Vladimirovna
- Gradoboeva Anastasija Evgen'Evna
- Sambuk Elena Viktorovna
- Smirnov Mikhail Nikolaevich
|
RU2386694C2 |
| THROMBOPOIETIN |
1994 |
- Iton Dehn L.
- De Sovazh Frederik Zh.
|
RU2245365C2 |
| ISOLATED DNA MOLECULE ENCODING HUMAN ERYTHROPOIETIN (VARIANTS), EXPRESSING PLASMID OR VIRAL DNA-VECTOR, GLYCOPROTEIN ERYTHROPOIETIN (VARIANTS) AND METHOD FOR ITS PREPARING, PHARMACEUTICAL COMPOSITION (VARIANTS), MAMMALIAN CELL LINE (VARIANTS) |
1985 |
|
RU2261276C2 |
| STRAIN OF YEAST PICHIA PASTORIS PS105(PBIG) AS PRODUCER OF BOVINE INTRACELLULAR IMMUNE INTERFERON, RECOMBINANT PLASMID DNA PBIG PROVIDING BIOSYNTHESIS OF BOVINE INTRACELLULAR IMMUNE INTERFERON AND METHOD FOR CONSTRUCTING RECOMBINANT PLASMID DNA PBIG |
2002 |
- Gradoboeva A.E.
- Padkina M.V.
- Parfenova L.V.
- Sambuk E.V.
- Smirnov M.N.
|
RU2231545C1 |
| YEAST STRAIN Pichia pastoris PS106(pHIG) AS PRODUCER OF HUMAN IMMUNE INTERFERON, RECOMBINANT PLASMID pHIG AND METHOD FOR ITS DESIGN |
2006 |
- Gradoboeva Anastasija Evgen'Evna
- Padkina Marina Vladimirovna
- Sambuk Elena Viktorovna
- Smirnov Mikhail Nikolaevich
|
RU2315806C1 |
| INTERFERON-LIKE PROTEIN ZCYTO21 |
2001 |
- Sheppard Pol O.
- Presnell Skott R.
- Foks Brajan A.
- Gilbert Tereza
- Kholdmen Betti A.
- Grant Frehnsis Dzh.
|
RU2292394C2 |
| RECOMBINANT PLAZMID DNA pART27INT6 AND METHOD OF OBTAINING ON ITS BASIS OF INBRED LINE OF TOBACCO PLANTS SYNTHESISING INTRACELLULAR GAMMA INTERFERON OF BULL |
2014 |
- Lutova Ljudmila Alekseevna
- Padkina Marina Vladimirovna
- Savel'Eva Natal'Ja Vladimirovna
- Emel'Janov Vladislav Vladimirovich
- Burlakovskj Mikhail Sergeevich
- Zobnina Anastasija Evgen'Evna
- Tkachenko Aleksandr Anatol'Evich
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RU2564115C1 |
