METHOD OF DETERMINING TOXICITY OF CHEMICAL SUBSTANCES IN AQUEOUS MEDIA Russian patent published in 1998 - IPC

FIELD: analytical methods in environmental monitoring. SUBSTANCE: invention is designed to control effluents of surface and industrial waste waters, for example, drilling fluids and also to estimate toxicity of newly-synthesized chemicals, etc. Sample of chemical substance is allowed to affect suspension of test-culture cells Paramecium Caudatum, after which changes in the test culture are determined by photometric technique. Toxicity of chemical substance is evaluated from decrease in moving activity of sample of the test-culture cell suspension (S_A) in presence of toxic agent as compared to the same quantity in pure (reference) suspension using the following expression: K_T= C_T/S_A where K_T is coefficient of toxicity of chemical substance, C_T concentration of toxic agent in the test-culture cell suspension. Decrease in moving activity of test-culture cells is determined from expression: S_A= (1-J_t/J_r)•100 where J_t is reference sample indication mean and J_r test sample indication mean. EFFECT: increased reliability and reproducibility of determinations. 2 dwg , 2 tbl