FUSED PROTEIN AND A METHOD OF ISOLATION OF THE FUSED PROTEIN Russian patent published in 1998 - IPC

FIELD: biology, medicine, biochemistry. SUBSTANCE: fused protein no occurring in nature and containing elongating peptide fragment covalently bound by its C-end with N-end of protein central part of the general formula (I): A-X-Y-(X¹-Y)_n-protein where A - H, Met and if A - H then protein is an analog of bGRF; if A - Met then protein is HIV-RNAase-4; X - Ala, Ser, Tyr, Val, Arg, Gly, Lys, Pro, Phe; Y - Ala, Ser, Thr, Pro; X′ - Ala, Gly, Ile, His, Lys, Phe, Tyr; n = 0-4; if n = 0 and A - H, Y - Ala, Ser, Thr. Method of isolation of fused protein of the formula (I) where A - Met; n = 4; X - Ala, Gly, Pro; Y - Ala, Pro; X′ - His; "protein" - HIV-RNAase involves column chromatography on Sepharose Pharmacia containing immobilized metal ions. Fused protein can be used for construction of drugs showing the prolonged effect. EFFECT: improved method of isolation. 9 cl, 6 tbl