FIELD: biotechnology, medicine. SUBSTANCE: genome DNA fragments from human fetal liver and the corresponding fragments to DNA providing high expression levels of erythropoietin are cloned in mammalian cells. Cell line CHO - a producer of recombinant erythropoietin (CHO EPO Cla 44.02-7) is obtained. As a result of expression of the cloned DNA fragments in mammalian cells O-glycosylated human erythropoietin is obtained at specific activity 200 000 U/mg, not less, and pharmaceutical composition using the indicated recombinant erythropoietin as an active component. EFFECT: improved method of preparing, increased specific activity of erythropoietin. 12 cl, 11 tbl, 12 dwg
| Title |
Year |
Author |
Number |
| METHOD OF HUMAN ERYTHROPOIETIN PREPARING |
1996 |
- Ehdvard Fritch
- Rodni M.Kh'Juvik
- Kennet Dzhokobs
|
RU2129613C1 |
| METHOD OF HUMAN ERYTHROPOIETIN PREPARATION |
0 |
- Edvard Fritch
- Rodni M.Khyuvik
- Kennet Dzhekobs
|
SU1801118A3 |
| METHOD FOR PREPARATION OF CDNA-CLONES, INCODING HUMAN ERYTHROPOIETIN |
0 |
- Edvard Fritch
- Rodni M.Khyuvik
- Kennet Dzhekobs
|
SU1672930A3 |
| METHOD OF EXPRESSION OF INTERESTING DNA SEQUENCE IN SEED CELL, DNA-STRUCTURE, EXPRESSION CASSETTE, ISOLATED REGULATORY SITE OF TRANSCRIPTION AND METHOD OF ALTERNATION OF SPECIFIC METABOLISM IN SEEDS |
1993 |
|
RU2148647C1 |
| FUSED POLYPEPTIDE ABLE TO PURPOSEFUL TRANSFER TO OILY BODY, CHIMERIC DNA-CONSTRUCTION, EXPRESSING CASSETTE |
1992 |
|
RU2201449C2 |
| METHOD OF PREPARING POLYPEPTIDE SHOWING PROPERTY OF HUMAN GAMMA-INTERFERON |
1988 |
- Dehvid V.Goeddel[Us]
- Patrik V.Grej[Us]
|
RU2092561C1 |
| RECOMBINANT DNA FRAGMENT THAT CODES SPECIFIC NITRILASE DETERMINING PLANT RESISTANCE TO HERBICIDES BASED UPON 3.5-DIHALOID-4-OXYBENZONITRILE; EXPRESSION VECTOR FOR RECOMBINANT DNA FRAGMENT (VARIANTS) |
1989 |
- Bernar Leruks[Fr]
- Bernar Peliss'E[Fr]
- Mishel' Lebrehn[Fr]
|
RU2106410C1 |
| RECOMBINANT PLASMID DNA PKEP-9 ENCODING HUMAN ERYTHROPOIETIN, STRAIN OF CULTURED CHINESE HAMSTER OVARY CELLS CHOPE-9 - A PRODUCER OF HUMAN ERYTHROPOIETIN |
1997 |
- Urmanova M.A.
- Shchelkunov S.N.
- Pozdnjakov S.G.
- Kolokol'Tsova T.D.
- Jurchenko N.D.
- Nesterov A.E.
- Shumakova O.V.
- Kostina N.E.
- Nechaeva E.A.
|
RU2118662C1 |
| MTS GENE, MUTATIONS OF THIS GENE AND METHODS OF DIAGNOSIS OF MALIGNANT TUMORS USING MTS GENE SEQUENCE |
1995 |
|
RU2164419C2 |
| RECOMBINATION PLT 9 PLASMIDE DNA WHICH CODES POLYPEPTIDE POSSESSING PROPERTIES OF HUMAN LYMPHOTOXIN, RECOMBINATION PLASMIDE PLT 16 DNA USED FOR CONSTRUCTING PLT 9 DNA, AND 'ESCHERICHIA COLI' BACTERIUM STRAIN - PRODUCER OF POLYPEPTIDE HAVING QUALITIES OF HUMAN LYMPHOTOXIN |
1988 |
- Korobko V.G.
- Dobrynin V.N.
- Filippov S.A.
- Chumakov A.M.
- Panina A.A.
- Boldyreva E.F.
- Nedospasov S.A.
- Shakhov A.N.
- Turetskaja R.L.
|
SU1561510A1 |
