Patenton — panteon of patents

(19)

RU

(11)

2 118 662

(13)

C1

(51)

IPC

(21) (22)

Application

97108266/13, 1997-05-20

(22)

Actual filing date

1997-05-20

(45)

Published

1998-09-10

(72)

Inventor

Urmanova M.A.Shchelkunov S.N.Pozdnjakov S.G.Kolokol'Tsova T.D.Jurchenko N.D.Nesterov A.E.Shumakova O.V.Kostina N.E.Nechaeva E.A.

(73)

Holder

Gosudarstvennyj Nauchnyj Tsentr Virusologii I Biotekhnologii

RECOMBINANT PLASMID DNA PKEP-9 ENCODING HUMAN ERYTHROPOIETIN, STRAIN OF CULTURED CHINESE HAMSTER OVARY CELLS CHOPE-9 - A PRODUCER OF HUMAN ERYTHROPOIETIN Russian patent published in 1998 - IPC

Abstract RU 2118662 C1

FIELD: molecular biology, biotechnology, genetic engineering. SUBSTANCE: invention relates to preparing recombinant plasmid vector pKEP-9 containing 8 116 nucleotide pairs (molecular mass is 5 mDa) that provides biosynthesis of human erythropoietin in cultured Chinese hamster ovary cells. Method of preparing involves fusion of Bst EII-Bgl II-fragment (2 383 nucleotide pairs) of plasmid pSY-Ep-gpt containing human erythropoietin structural gene into Smaq I-site of vector plasmid pNut (5 733 nucleotide pairs) containing activated murine metallothionein promoter MT-1 and dihydrofolate reductase gene under control of early promoter SV-40. Recombinant strain CHOpE-9 is obtained after transformation of cells CHO-KIdhfr with constructed recombinant plasmid pKEP-9 followed by culturing on growth medium and selection of colonies showing resistance to methotrexate and hybridizing with pKEP-9 DNA. Cells of new strain under conditions of stationary culturing stably produce human erythropoietin in cultural fluid at the yield 400 UA/ml medium. EFFECT: plasmid and cells indicated above, increased yield of erythropoietin. 2 cl, 3 ex, 2 dwg

Similar patents RU2118662C1

Title Year Author Number
STRAIN CHO OF THE CULTURED CHINESE HAMSTER CELLS - A PRODUCER OF HUMAN ERYTHROPOIETIN 1995
  • Zelenin M.G.
  • Kramerova I.A.
  • Kolobkov S.L.
  • Filjakina N.S.
  • Filatov I.A.
 RU2089611C1
RECOMBINANT PLASMID DNA P7SHSHIS FOR PRODUCTION OF DIMERIC SINGLE-STRANDED ANTIBODY AGAINST TICK-BORNE ENCEPHALITIS VIRUS AND STRAIN OF ESCHERICHIA COLI AS PRODUCER OF DIMERIC FORM OF SINGLE-STRANDED ANTIBODY AGAINST TICK-BORNE ENCEPHALITIS VIRUS 2000
  • Nikolenko G.N.
  • Tikunova N.V.
  • Protopova E.V.
  • Loktev V.B.
  • Karpenko L.I.
  • Konovalova S.N.
  • Il'Ichev A.A.
 RU2190017C2
RECOMBINANT PLASMID DNA PQ-F35 ENCODING FUSED POLYPEPTIDE F35 SHOWING ANTIGENIC AND IMMUNOGENIC PROPERTIES OF MARBURG VIRUS PROTEIN VP 35, METHOD OF ITS PREPARING AND STRAIN OF BACTERIUM ESCHERICHIA COLI - SUPERPRODUCER OF RECOMBINANT POLYPEPTIDE F35 1998
  • Sorokin A.V.
  • Razumov I.A.
  • Kazachinskaja E.I.
  • Kachko A.V.
  • Ivanova A.V.
  • Mishin V.P.
  • Bukreev A.A.
  • Belanov E.F.
  • Loktev V.B.
  • Netesov S.V.
 RU2144565C1
RECOMBINANT DNA PLASMID PGSDE1 ENCODING TICK-BORNE ENCEPHALITIS VIRUS PROTEIN E AND STRAIN OF ESCHERICHIA COLI - PRODUCER OF TICK-BORNE ENCEPHALITIS VIRUS PROTEIN E 1997
  • Netesova N.A.
  • Belavin P.A.
  • Malygin Eh.G.
  • Rukavishnikov M.Ju.
 RU2136754C1
RECOMBINANT PLASMID DNA PTBI-HBSAG COMPRISING CHIMERIC GENE TBI-HBSAG UNDER CONTROL OF SMALLPOX VACCINE PROMOTER P7,5K AND STRAIN OF RECOMBINANT SMALLPOX VACCINE INDUCING IMMUNE RESPONSE AGAINST HIV AND HUMAN HEPATITIS B IN ANIMAL BODY 2001
  • Shchelkunov S.N.
  • Rjazankin I.A.
  • Ageenko V.A.
  • Ignat'Ev G.M.
  • Babkin I.V.
 RU2194075C2
VIRAL GENOME FRAGMENT OF CHICKEN K STRAIN VARIOLA WHICH CONTAINS THYMIDINE KINASE GENE, pTK1FPV AND pTK2FPV RECOMBINANT PLASMID DNAs WHICH CONTAIN THIS SEQUENCE, AND pINTFPV1 RECOMBINANT PLASMID DNA WHICH IS ABLE TO INTEGRATE INTO VIRAL GENOME OF CHICKEN VARIOLA 1999
  • Susloparov M.A.
  • Pljasunov I.V.
  • Bakhtina M.M.
  • Susloparov I.M.
  • Makhova N.M.
  • Lokteva L.A.
 RU2180352C2
RECOMBINANT PLASMID DNA PTLS CONTAINING GENE OF SINGLE-STRANDED ANTIBODY AGAINST TICK-BORNE ENCEPHALITIS VIRUS AND STRAIN OF BACTERIUM ESCHERICHIA COLI AS PRODUCER OF SINGLE-STRANDED ANTIBODIES AGAINST TICK-BORNE ENCEPHALITIS VIRUS 1998
  • Nikolenko G.N.
  • Tikunova N.V.
  • Golovin S.Ja.
  • Protopopova E.V.
  • Loktev V.B.
  • Il'Ichev A.A.
  • Deev S.M.
 RU2158309C2
RECOMBINANT PLASMID DNA PGGF-8 ENCODING POLYPEPTIDE SHOWING PROPERTIES OF HUMAN GRANULICYTIC COLONY-STIMULATING FACTOR AND THE STRAIN OF BACTERIUM ESCHERICHIA COLI - PRODUCER OF POLYPEPTIDE SHOWING PROPERTIES OF HUMAN GRANULOCYTIC COLONY-STIMULATING FACTOR 1996
  • Korobko V.G.
  • Shingarova L.N.
  • Petrovskaja L.E.
  • Petrenko L.A.
  • Pustoshilova N.M.
 RU2113483C1
RECOMBINANT PLASMID DNA PUL 44 HCMV DETERMINING EXPRESSION OF HUMAN CYTOMEGALOVIRUS GENE UL 44 FRAGMENT ENCODING IMMUNODOMINANT SITE OF DNA-BINDING PROTEIN P 52 (ICP 36) IN ESCHERICHIA COLI BACTERIUM CELLS 1998
  • Susloparov M.A.
  • Susloparov I.M.
  • Pljasunov I.V.
 RU2151800C1
RECOMBINANT PLASMID DNA PURVGF DETERMINING SYNTHESIS OF SMALLPOX VIRUS STRAIN L-IVP GROWTH FACTOR TOGETHER WITH ESCHERICHIA COLI BETA-GALACTOSIDASE AND STRAIN OF BACTERIUM ESCHERICHIA COLI CONTAINING RECOMBINANT PLASMID DNA PURVGF - A PRODUCER OF SMALLPOX VIRUS STRAIN L-IVP GROWTH FACTOR 1993
  • Petrov V.S.
  • Belavin P.A.
  • Cheshenko N.V.
  • Raspopin V.V.
  • Patrushev N.A.
  • Krendel'Shchikov A.V.
  • Malygin Eh.G.
 RU2089612C1

RU 2 118 662 C1

Authors

Urmanova M.A.

Shchelkunov S.N.

Pozdnjakov S.G.

Kolokol'Tsova T.D.

Jurchenko N.D.

Nesterov A.E.

Shumakova O.V.

Kostina N.E.

Nechaeva E.A.

Dates

1998-09-10—Published

1997-05-20—Filed

download Download PDF read Similar patents