METHOD OF HUMAN ERYTHROPOIETIN PREPARING Russian patent published in 1999 - IPC

Abstract RU 2129613 C1

FIELD: genetic engineering. SUBSTANCE: invention relates to human erythropoietin gene cloning. Mammalian cells transformed by the recombinant vector are cultured in suitable medium. Vector has DNA sequences given in tables 3 and 4. DNA sequence can be introduced into vector containing DNA of bovine papilloma virus. Mammalian cells are lines COS, CHO, C127 or 3T3. Cultural acid can contain fetal serum. Obtained erythropoietin is separated from cells and medium. Method ensures to obtain the cloned human erythropoietin genes exhibiting highly levels of expression. EFFECT: high levels of expression of the cloned erythropoietin genes. 6 cl, 11 tbl, 12 dwg, 15 ex

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RU 2 129 613 C1

Authors

Ehdvard Fritch

Rodni M.Kh'Juvik

Kennet Dzhokobs

Dates

1999-04-27—Published

1996-03-12—Filed