FIELD: virology. SUBSTANCE: method involves reaction monitoring and result recording on the basis of solid-phase immunoenzyme analysis. Biological materials: 10% suspension of organs to be analyzed, blood, cultural materials infected with Rift Valley fever virus. Biological materials are sorbed in microtitrating plate wells followed by sorption of specific IgG-biotin. After washing out streptavidin and biotin-β--galactosidase taken at ratio 2: 1 or 1:1, respectively, are sorbed. A set for detection of Rift Valley fever virus in biological material has an antigen, conjugates and streptavidin additionally. A set has a specific IgG-biotin and biotin-β--galactosidase as conjugates and antigens: control antigen and specific antigen to Rift Valley fever virus. A set has these preparations placed into ampoule (volume is 0.5 ml). EFFECT: enhanced sensitivity of reaction. 2 cl, 2 tbl, 1 ex
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