FIELD: pharmaceutical industry. SUBSTANCE: invention relates to methods of isolation of biologically active substances from plant raw. For preparing 20-hydroxyecdysone from ecdysone a mixture of ecdysteroids in water is passed through hydrophobic sorbents up to saturated capacity by ecdysone in eluate at inlet and outlet from sorbent. Then aqueous solution of organic solvents are eluted through a sorbent layer. Solvent is evaporated, products are purified by HPLC-chromatography or recrystallization. EFFECT: increased output of process, retained stage numbers. 4 ex
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