FIELD: microbiological industry. SUBSTANCE: culture is grown on medium with precursors of enzyme taking part in metabolic processes and obtained biomass is frozen at temperature range from -9 C to -20 C. Then biomass is frozen out and grown again on medium with enzyme precursors up to required cell titer value. Method increases the yield of products of microbiological synthesis in microorganism cultures growing. EFFECT: improved method of preparing, increased yield. 2 cl, 6 ex
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