FIELD: biotechnology, biochemistry. SUBSTANCE: invention relates to production of enzymes used in the problem of universal donor carriers and erythrocytes showing specificity by the group properties and used for emergency transfusion help. Enzyme α-N-acetyl-galactose aminidase is able to remove α-1,3-bound residues of N-acetylgalactoseamine from glycoproteins of blood group substances A (II) and convert their to substances of the group 0(H). The strain of bacterium Flavobacterium sp. (VKM B-2137D) is isolated from sea bottom by method of direct sowing. After culturing at 25 C for 24-30 h the strain makes biosynthesis of α-N-acetylgalactose aminidase on the nutrient medium containing the following components, g/l: bactopeptone 5.0; glucose 1.0; K2HPO4 0.2; yeast extract (Difco) 2.0; distilled water 250 ml and sea water 750 ml (pH 7.5). The preparation of α-N-acetylgalactose aminidase is isolated from biomass by treatment of cells by ultrasonic oscillation followed by enzyme purification by ion-exchange chromatography on DEAE-Toyoperl 650-M, gel-filtration on Sepharose CL-6B and rechromatography on DEAE-Toyoperl 650-M. The yield of the preparation of α-N-acetylgalactose aminidase is 20% of its content in crude extract at specific activity 22 U/mg of protein. The enzyme shows an optimum activity at pH 7.2, retains 100% of activity at heating at 50 C for 10 min, its molecular mass is 84 kDa. The enzyme shows the unique ability to inactivate the group specificity of human blood erythrocytes of the group A (II). EFFECT: strain of bacterium indicated above, improved method of enzyme preparing, high yield and specific activity, valuable properties. 3 tbl, 1 dwg, 1 ex
