FIELD: medicine.
SUBSTANCE: method involves homogenization of actinia Heteractis crispa, ethanol extraction of polypeptides, polychrome-1 hydrophobic chromatography, following purification of an end product by ion-exchange chromatography and reverse phase HPLC. Polychrome-1 hydrophobic chromatography is conducted in a static version, while ion-exchange chromatography is conducted on Cellulose CM-32.
EFFECT: end product yield higher in 12 times.
2 dwg, 1 tbl, 1 ex
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Authors
Dates
2011-04-10—Published
2009-11-30—Filed